Gαqbinds to p110α/p85α phosphoinositide 3-kinase and displaces Ras
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چکیده
منابع مشابه
Binding of Ras to Phosphoinositide 3-Kinase p110α Is Required for Ras- Driven Tumorigenesis in Mice
Ras proteins signal through direct interaction with a number of effector enzymes, including type I phosphoinositide (PI) 3-kinases. Although the ability of Ras to control PI 3-kinase has been well established in manipulated cell culture models, evidence for a role of the interaction of endogenous Ras with PI 3-kinase in normal and malignant cell growth in vivo has been lacking. Here we generate...
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domain-containing adaptor subunit, p85 , p85 , or p55 , Background: Type I phosphoinositide 3-kinases are rederived from three possible genes (or their splice varisponsible for the hormone-sensitive synthesis of the ants). Class IA can be activated directly by the associalipid messenger phosphatidylinositol(3,4,5)-trisphostion of the SH2 domains of their regulatory subunits phate. Type IA and I...
متن کاملRas isoforms vary in their ability to activate Raf-1 and phosphoinositide 3-kinase.
Ha-, N-, and Ki-Ras are ubiquitously expressed in mammalian cells and can all interact with the same set of effector proteins. We show here, however, that in vivo there are marked quantitative differences in the ability of Ki- and Ha-Ras to activate Raf-1 and phosphoinositide 3-kinase. Thus, Ki-Ras both recruits Raf-1 to the plasma membrane more efficiently than Ha-Ras and is a more potent acti...
متن کاملCrystal structure and functional analysis of Ras binding to its effector phosphoinositide 3-kinase gamma.
Ras activation of phosphoinositide 3-kinase (PI3K) is important for survival of transformed cells. We find that PI3Kgamma is strongly and directly activated by H-Ras G12V in vivo or by GTPgammaS-loaded H-Ras in vitro. We have determined a crystal structure of a PI3Kgamma/Ras.GMPPNP complex. A critical loop in the Ras binding domain positions Ras so that it uses its switch I and switch II region...
متن کاملCrystal Structure and Functional Analysis of Ras Binding to Its Effector Phosphoinositide 3-Kinase γ
suppression of apoptosis (Franke et al., 1997), reorganization of the actin cytoskeleton (Rodriguez-Viciana et al., 1997), cardiac myocyte growth (Shioi et al., 2000), glycogen synthase stimulation by insulin (reviewed in Shepherd et al., 1998), TNFa-mediated neutrophil primMichael E. Pacold,* Sabine Suire,† Olga Perisic,* Samuel Lara-Gonzalez,* Colin T. Davis,* Edward H. Walker,* Phillip T. Ha...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 2006
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj20051493